Product Description
AtaGenix offers high-purity Endo S (Endoglycosidase S), a reliable and efficient enzymatic solution for glycoprotein analysis and immunology research. Endo S, derived from Streptococcus pyogenes, is a highly specific endoglycosidase that cleaves N-linked glycans from the chitobiose core of the heavy chain in wild-type IgG, providing precise and efficient enzymatic activity for various applications.
Product Advantages
- High Specificity: Precisely removes N-linked glycans at the chitobiose core of IgG heavy chains, avoiding nonspecific reactions.
- High Purity and Activity: Validated by SDS-PAGE, with purity >95% and excellent stability and enzymatic activity.
- No Contaminant Enzymes: Free from exoglycosidase and protease contamination, ensuring reliable experimental results.
- Convenient Purification: Features an N-terminal His tag for easy removal via affinity chromatography.
Applications
- IgG glycan analysis and research
- Glycoprotein structural and functional studies
- Biotechnology and pharmaceutical development
- Immunology and molecular biology experiments
Product Specifications
| Attribute | Details |
|---|---|
| Product Name | Endo S (Endoglycosidase S) |
| Catalog Number | ATE00017 |
| Storage | Store at -20°C, shelf life: 12 months |
| Shipping | Shipped on blue ice |
| Form | Liquid |
| Buffer | PBS (pH 7.4), 50% glycerin |
| Host | E. coli |
| Cleavage Site | Cleaves N-linked glycans at the chitobiose core of wild-type IgG heavy chains. |
| Purity | >95% (validated by SDS-PAGE) |
| Specific Activity | 40,000 U/ml |
| Unit Definition | One unit is defined as the amount of enzyme required to remove 95% of N-linked glycans from 5 µg of native mouse IgG in a total reaction volume of 10 µl at 37°C for 1 hour. |
| Reaction Conditions |
1X Buffer:
|
| Tag | N-terminal His Tag |
About AtaGenix
AtaGenix produces Endo S through recombinant expression in E. coli, using rigorous purification processes to ensure high purity, activity, and stability. Our product is widely applied in glycoprotein research, immunology, and biopharmaceutical development, making it an ideal tool for scientists worldwide.
Choose AtaGenix to accelerate your research and innovation!
