AtaGenix Laboratories
AtaGenix Laboratories
Human MHC is known as Human Leukocyte Antigen (HLA). Human MHC can be divided into MHC I, MHC II and MHC III. MHC I is composed of an alpha chain that spans the cell membrane and an extracellular β2- microglobulin (B2M) attached to this chain. The entire molecule consists of four regions, three of which are in the α chain (α1, α2, α3) and the β2-microglobulin forms the fourth region. The α1 and α2 located outside the cell form a groove in which the polypeptide can bind. Most T lymphocytes express a single, highly specific antigen receptor, TCR, on their surface, which, in the presence of an antigen-presenting cell (APC), can bind directly to the MHC I- peptide complex and initiate a CD8-specific immune response. The TCR has a relatively low affinity for monomeric MHC I- polypeptide complexes and a rapid mismatch rate, while its affinity for MHC Ipolypeptide tetramers is greatly enhanced.
We leverage diverse expression systems, including but not limited to E. coli, yeast, insect cells, and mammalian cells, to ensure the efficient production of target proteins. Utilizing advanced protein refolding techniques, HLA, β2M, and peptide fragments are accurately assembled into stable MHC-peptide complexes.
To further enhance functionality, we incorporate innovative SA active proteins, boosting TCR recognition and improving the affinity between the complex and T-cell receptors. Our process follows rigorous high-standard purification protocols, employing advanced methods such as affinity chromatography and molecular sieving to eliminate impurities, ensuring the final product's purity and functionality.
Contact Us
+86-27-87001869
info@atagenix.com
Building C, R & D Building, No. 666, Shendun 4th Road, Donghu New Technology Development Zone, Wuhan
