Product Description
AtaGenix provides high-purity Recombinant Bovine Enterokinase (rb-EK), a reliable and efficient enzymatic tool for protein research. Enterokinase is a highly specific serine protease that precisely recognizes the Asp-Asp-Asp-Asp-Lys (DDDDK) sequence and cleaves at the C-terminal side of Lys, rapidly removing N-terminal fusion tags to release target proteins.
Product Advantages
- High Specificity: Accurately cleaves the DDDDK sequence, ensuring precise enzymatic reactions and preventing nonspecific degradation.
- High Efficiency: One milligram of recombinant bovine enterokinase can cleave at least 1.5 grams of fusion protein, making it suitable for large-scale protein production.
- Broad Applicability: Ideal for genetic engineering, biochemistry, molecular biology, and biopharmaceutical research.
- Convenient Purification: C-terminal His tag enables easy removal via metal affinity chromatography, ensuring high-purity target proteins.
Applications
- Removal of N-terminal tags from fusion proteins
- Protein purification and functional studies
- Biotechnology and pharmaceutical development
Product Specifications
| Attribute | Details |
|---|---|
| Product Name | Recombinant Bovine Enterokinase (rb-EK) |
| Catalog Number | ATE00002 |
| Storage | Store at -20°C, shelf life: 12 months |
| Shipping | Shipped on blue ice |
| Form | Liquid |
| Buffer | 50 mM Tris-HCl, 50% Glycerol, pH 8.0 |
| Host | Yeast |
| Molecular Weight | 26.2 kDa |
| Purity | >95% (validated by SDS-PAGE) |
| Activity | 2 U/µl |
| Specific Activity | >30 KU/mg (1 mg rb-EK can cleave at least 1.5 g fusion protein). |
| Unit Definition | One unit is defined as the amount of enzyme required to cleave 50 µg of a fusion protein containing the cleavage site in 16 hours at 25°C, in 50 mM Tris-HCl (pH 8.0). |
| Reaction Conditions | Cleavage at 25°C for 16 hours |
| Tag | C-terminal His Tag |
About AtaGenix
AtaGenix's recombinant bovine enterokinase is produced using stringent manufacturing and purification processes, ensuring high purity, stability, and efficiency. Widely applied in protein engineering and genetic engineering, our enterokinase products provide reliable support for research experiments, making them ideal for laboratory and industrial applications.
Choose AtaGenix to accelerate your research and innovation!
