Product Description
AtaGenix offers high-purity TEV Protease, a reliable enzymatic solution for protein research. Derived from Tobacco Etch Virus (TEV) Nla, this recombinant protease exhibits exceptional site specificity, recognizing the seven-amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln↓Gly) and cleaving precisely between Gln and Gly. It efficiently removes affinity tags, such as GST or other fusion tags, ensuring precise and effective protein preparation.
Product Advantages
- High Specificity: TEV Protease precisely recognizes the seven-amino acid sequence, ensuring accurate cleavage and avoiding nonspecific cuts.
- Broad Reaction Conditions: Retains high activity over a wide range of pH (6.0–8.5) and temperatures (4–30°C), making it versatile for various experimental setups.
- Convenient Purification: Features an N-terminal GST tag, allowing easy removal using glutathione affinity chromatography, simplifying workflows.
Applications
- Removal of affinity tags (e.g., GST) from fusion proteins
- Protein purification and functional studies
- Flexible enzymatic cleavage for various experimental conditions
Product Specifications
| Attribute | Details |
|---|---|
| Catalog Number | ATE00001 |
| Product Name | TEV Protease |
| Storage | Store at -20°C, shelf life: 12 months |
| Shipping | Shipped on blue ice |
| Form | Liquid |
| Buffer | 25 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, 50% glycerol, pH 8.0 |
| Host | E. coli |
| Molecular Weight | 50 kDa |
| Purity | >95% (validated by SDS-PAGE) |
| Specific Activity | 17 U/µl |
| Unit Definition | One unit is defined as the amount of enzyme required to cleave >85% of 3 µg of substrate at 30°C, pH 8.0, in 1 hour. |
| Reaction Conditions | 1× TEV Protease Buffer (50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, 1 mM DTT, pH 8.0); cleave at 30°C for 1 hour. |
| Tag | N-terminal GST Tag |
About AtaGenix
AtaGenix produces TEV Protease through recombinant expression in E. coli, ensuring high purity and stability through rigorous purification processes. Widely used in protein engineering, tag removal, and other biotechnological applications, our TEV Protease is an ideal choice for your laboratory needs.
Choose AtaGenix to accelerate your research and innovation!
