Product Description
AtaGenix offers high-purity SUMO Protease, an efficient and reliable enzymatic tool for protein research. SUMO Protease precisely recognizes SUMO-tagged proteins containing the 100-amino acid SUMO sequence and cleaves the bond between SUMO and the fusion protein. Its extended recognition sequence provides superior specificity compared to traditional EK or TEV proteases, reducing nonspecific cleavage interference.
Product Advantages
- High Specificity: The long recognition sequence ensures accurate SUMO tag cleavage even in complex samples.
- Wide Reaction Conditions: Stable activity across temperatures from 4–30°C and pH ranges of 7.0–9.0.
- Convenient Purification: Features a His tag for easy removal using affinity chromatography, ensuring high-purity target proteins.
- Strong Compatibility: Performs effectively in both TBS (pH 8.0) and PBS (pH 7.5) buffers, making it adaptable to various experimental systems.
Applications
- Efficient removal of SUMO fusion tags
- Accurate purification of target proteins
- Protein structure and functional studies
Product Specifications
| Attribute | Details |
|---|---|
| Catalog Number | ATE00004 |
| Product Name | SUMO Protease |
| Storage | Store at -20°C, shelf life: 12 months |
| Shipping | Shipped on blue ice |
| Form | Liquid |
| Buffer | 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 50% Glycerol |
| Host | E. coli |
| Molecular Weight | 26.55 kDa |
| Purity | >95% as determined by SDS-PAGE |
| Specific Activity | 10 U/µl |
| Unit Definition | One unit is defined as the amount of enzyme required to cleave >85% of 2 µg substrate protein in 1 hour at 30°C. |
| Reaction Conditions | React at 4°C for 16 hours under pH 8.0 conditions. |
| Tag | His Tag |
Why Choose AtaGenix?
AtaGenix's SUMO Protease delivers superior cleavage performance and robust technical support, helping researchers optimize workflows and enhance research efficiency. Choose AtaGenix to drive innovation in your protein research!
