Product Description
AtaGenix offers high-purity Rhinovirus 3C Protease, a reliable enzymatic tool for efficient and precise protein cleavage. Derived from human rhinovirus, 3C Protease specifically cleaves peptide bonds at the Gln-Gly site, recognizing the sequence Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro (LEVLFQ↓GP). With a molecular weight of approximately 22 kDa, this protease ensures high specificity and minimizes nonspecific degradation.
Product Features
- High Specificity: Precisely cleaves fusion proteins containing the 3C cleavage site, avoiding nonspecific degradation.
- Exceptional Bioactivity: Ensures reliable experimental results, supporting advanced research applications.
- GST-Tagged for Easy Removal: Simplifies purification workflows by allowing quick separation from the reaction system using glutathione affinity chromatography.
Applications
- Rapid removal of fusion protein tags
- Efficient cleavage of protein samples
- Optimization of protein purification workflows
Product Specifications
| Attribute | Details |
|---|---|
| Catalog Number | ATE00003 |
| Specifications | 100 U / 500 U / 500 U × 2 |
| Storage | Store at -20°C, shelf life: 12 months |
| Shipping | Shipped on blue ice |
| Form | Liquid |
| Buffer | 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 50% glycerol |
| Host | E. coli |
| Cleavage Site | Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro (LEVLFQ↓GP) |
| Molecular Weight | 47.38 kDa |
| Purity | >95% as determined by SDS-PAGE |
| Activity | 500 U/ml |
| Specific Activity | 500 U/mg |
| Unit Definition | One unit is defined as the amount of enzyme required to cleave 100 µg of substrate with >95% efficiency at 4°C for 16 hours. |
| Reaction Conditions | Cleavage buffer: 50 mM Tris-HCl (pH 7.0, 25°C), 150 mM NaCl, 1 mM EDTA, 1 mM DTT; react at 4°C for 16 hours. |
| Tag | N-terminal GST Tag |
About AtaGenix
As a leading provider of protein and antibody development services, AtaGenix specializes in high-quality research tools. Our Rhinovirus 3C Protease offers exceptional precision and usability, making it an indispensable tool for protein engineering, fusion protein research, and other biotechnological applications.
For more information or technical support, contact our customer service team.
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